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KMID : 0364820140500020164
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.164 ~ p.168
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Diversity Analysis for Archaeal amoA Gene in Marine Sediment of Svalbard, Arctic Circle
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Park Soo-Je
Rhee Sung-Keun
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Abstract
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The ecosystem of the Arctic region has been increasingly affected by global warming. Archaeal ammonia
monooxygenase alpha subunit coding gene (amoA) which is a key enzyme for nitrification was used to investigate
the effect of runoff water of ice melt on microbial community of nitrogen cycle. The archaeal amoA genes at coastal
area of Svalbard, Arctic region were PCR-amplified and sequenced after clone library construction. Analysis of
archaeal amoA gene clone libraries suggested that the station 188 which is in the vicinity to the area of runoff water
harbor lower ammonia-oxidizing archaeal diversity than the station 176 and 184. The average amino acid sequence
identity within all archaeal amoA gene clones was 94% (with 91% nucleotide sequence identity). While all the
clones of the station 188 were affiliated with Nitrosoarchaeaum clade containing strains isolated from low-salinity
and terrestrial environments, about 45% of total clones of the station 176 and 184 were related to marine
Nitosopumilus clade. Interestingly, other typical archaeal amoA gene clones of thaumarchaeal I.1b clade frequently
retrieved from terrestrial environments was identified at station 188. Microbial community of nitrogen cycle in
marine sediment might be affected by input of sediments caused by runoff glacier melt waters.
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KEYWORD
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amoA, ammonia-oxidizing archaea, arctic, diversity, marine sediment
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